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the supernatants containing total proteins were collected, and the protein concentration was quantified using a protein assay dye (Bio-Rad)

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Red flag symptoms that necessitate same-day medical contact include: Blood in stools (visible red blood or black, tarry stools) Severe abdominal pain that is persistent, severe, or radiating to the back (which may indicate pancreatitis, a rare but serious side effect) Signs of dehydration : decreased urination, dark urine, dizziness upon standing, extreme thirst, or confusion Fever above 100.4F (38C) accompanying diarrhea Pain in the upper right abdomen , possibly with fever or yellowing of skin/eyes (signs of gallbladder disease, which can occur with GLP-1 medications) Inability to keep down fluids due to concurrent vomiting If you experience severe vomiting or diarrhea leading to dehydration, you may need to temporarily pause your medication until speaking with your healthcare provider, as severe dehydration can lead to acute kidney injury

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