Briefly, to separate epidermis from dermis, whole skin from mice at postnatal day (P)0-P3 were incubated in a solution of 5 mg/ml Dispase II (#D4693, Sigma-Aldrich, USA) diluted in RPMI medium (Gibco, USA) supplemented with 10% fetal calf serum (FCS, #S0615, Sigma-Aldrich, USA), penicillin (100 U/ml), streptomycin (100 g/ml), 100 nM sodium pyruvate, and 10 mM non-essential amino acids (all Gibco, USA) at 4 C overnight
Confounding factors must also be considered
The potency might be half of what it was when it left the manufacturer
While you may be celebrating the pounds dropping on the scale, rapid medication-induced weight loss can lead to unexpected changes in your facial appearance
For example, diets rich in fiber promote the growth of beneficial bacteria, thereby increasing the production of SCFAs, which have been shown to exert antidepressant effects [506, 507]