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The contributing factors include: Facial fat loss : Weight loss affects fat throughout the body, including the face, leading to a loss of volume and support under the skin

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doi: 10.1038/s41598-023-27586-4 212

8 In our association analysis HOMA-IR and HOMA2-IR were both associated with GLP-1 z-score in boys and glucagon z-scores in girls

For reproducibility, investigators pre-register endpoints, include blinding and randomization, and document supplier, lot, and COA to maintain traceability across cohortsan approach that supports transparent reporting while keeping the framing strictly preclinical and non-therapeutic