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For the underlying technique how to add the diluent slowly down the vial wall, why you never shake it, and how to store the solution afterward see the full peptide reconstitution guide and the companion note on how much bacteriostatic water to use

3 Journal of Clinical Medicine

-arrestin 2 recruitment HEK293T cells (3.2 10 6 cells/10 cm plate) were grown for 24 h before transiently transfection with 15 g plasmid containing GIPR, GLP-1R or GCGR tagged with Rluc8 and -arrestin 2 with a Venus-tag at the N terminus at a ratio of 1:9 by Lipofectamine 3000 transfection reagent

Statistical analysis Instrumental data were analyzed using IBM SPSS Statistics software

D/C beta-lactam-resistant penicillin (flucoxacillin, dicloxacillin, cloxacillin, nafcillin)