In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min
clag9: a cytoadherence gene in Plasmodium falciparum essential for binding of parasitized erythrocytes to CD36
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The pre-stained biofilm was then allowed to dry for 15 min at 37C, after which it was dissolved using 80% v/v ethanol and transferred into new 96-well plate for biomass quantification at OD 550 nm using a Tecan plate reader (Infinite M1000 pro)