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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

Classical behavior tests confirmed depressive behavior like forced swim test (FST) [32,33,34, 36, 38,39,40, 42], tail suspension test (TST) [32,33,34, 36, 38, 39], sucrose preference test (SPT) [35, 38,39,40, 42], and exploratory activity in the open field test (OFT) [32,33,34, 36, 38, 40]

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Liang S, Gu Y