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The medication may be allowed to reach room temperature before administration if stored under refrigeration, as cold injections may be more painful and could potentially affect absorption kinetics

Due to chemisorption, the sulfhydryl groups in glutathione can combine with silver nanoparticles to produce AgS bonds, which attach to the silver nanoparticles surface and generate a large number of hot spots, resulting in the enhancement of the infrared signal intensity of glutathione

Both groups also underwent the same exercise programme

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The caco-2 cell line was purchased from the American Type Culture Collection (Manassas, VA, USA)