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Notably, Fasn and Scd1 are target genes of SREBP1 25 , and Cpt-1 and Acox1 are PPAR/PGC-1 regulated genes 26

C-met binding was examined at two HGF-doses (250 ng/ml and 500 ng/ml in PBS) by sandwich ELISA-like assay on recombinant c-met-Fc chimera as in Figure 1e, and significant changes in optical absorbance subtracted by a baseline level (negative control without HGF, bar 1 ) were depicted in Figure 2a (left column)

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0.22 m) immediately prior to use Aliquot and store reconstituted solutions at 20 C (or lower) to minimise degradation Avoid repeated freezethaw cycles Specifications Summary Purity (HPLC): 99 % Appearance: White to off-white lyophilised powder Peptide Type: Synthetic growth hormone secretagogue (GHSR-1a agonist) Molecular Formula: C38H49N9O5 Molecular Weight: ~711.85 g/mol Solubility: Water, buffered aqueous solutions Storage: 20 C, desiccated, protected from light Pack Size: 5 mg Precautions & Notes Experimental behaviour of ipamorelin is influenced by receptor expression, concentration, and exposure duration Unlike GHRP-6 and GHRP-2, ipamorelin does not stimulate ACTH or cortisol release at GH-stimulating concentrations Buffer composition, pH, and ionic strength may affect peptide stability and assay performance Appropriate controls are recommended to distinguish receptor-specific from non-specific effects Intended strictly for laboratory research use

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