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The supernatant was transferred to LCMS vials, and the pellet was lysed by 20 mM NaOH and protein levels were detected for normalization

However, long-term use of corticosteroids is associated with adverse effects, including immune suppression and increased susceptibility to infections, which limits their clinical utility (Brahmer et al., 2018)

[15], age did not correlate with muscle cramps, suggesting that senile muscle mass reduction is probably not a contributing factor to cirrhotic cramps