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Primary antibodiesGAPDH (60004-1-Ig, Proteintech, Wuhan, China), CPT1B (DF3904, Affinity, USA), OCTN2 (16331-1-AP, Proteintech, Wuhan, China), Akt (60203-2-Ig, Proteintech, Wuhan, China), p-Akt (80642-1-rr, Proteintech, Wuhan, China), and PGC-1 (Ab 313559, Abcam, Shanghai, China)were incubated at 4 C overnight