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Metformin (Met) is acknowledged by the World Health Organization (WHO) as an indispensable pharmaceutical agent for the management of diabetic patients 7

Due to the relatively rapid hydrolysis rate of the imine linker between PEG and T ECM -NS, about 85% of PEG was removed from PEG-T ECM -NS within 2 h

cDNA was synthesized using SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen, Grand Island, NY), according to the manufacturer's guidelines