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Elevated homocysteine in the blood, otherwise known as homocystinuria, if left untreated, can result in abnormal blood clotting, brittle bones, and nearsightedness, and increased risk of having a child with a neural tube defect (spina bifida)

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Vitamin B12 malabsorption in patients with limited ileal resection

To detect SyeA with western blotting, protein lysate samples were mixed with 6 SDS dye, boiled at 100 C for 10 min and run on a 12% acrylamide gel at 120 V for 1 h