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Inhibition of nuclear translocation and transacting function

Quantitative PCR RNA was extracted from bEnd.3 cells using the RNeasy mini kit from Qiagen (Valencia, CA) as per manufacturers protocols

This has been shown to result in decreased production of pro-inflammatory cytokines such as Tumor Necrosis Factor alpha (TNF-), interleukin 6 (IL-6), interleukin 8 (IL-8), and interferon gamma (IFN-), and inflammation markers such as cyclooxygenase 2 (COX2) 26,45

This is due to the fact that glutathione is required for the methylation of vitamin B12, and thus the continued formation of related products (Xia et al., 2004)

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