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These mechanisms enhance FoxO-driven expression of both classical and noncanonical E3 ligases, robustly activating UPS (Figure 8 shows the ROS-mediated activation of FoxO signaling in muscle protein degradation

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The metabolites were separated by applying a temperature gradient: the initial temperature was 60 C, increased to 300 C at 15C/min over 16 min and held for 4 min