Made from amino acids in the liver, glutathione is an antioxidant that promotes tissue repair and weakens free radicals
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Materials and methods Reagents and antibodies The following antibodies were used for western blotting: anti-NLRP3(15101S, Cell Signaling Technology), anti-GSDMD (NBP2-33422, Novus Biologicals), anti-IL-1(ab234437, Abcam), anti-IL-18 (ab207323, Abcam), anti-CD31(AP3628, R&D systems), anti-VEGF (sc-7269, Santa Cruz), anti-CCL11(ab133604, Abcam), anti--actin (4970S, Cell Signaling Technology)
Thiobarbituric acid (TBA) (40 mM, 1.5 mL) was added to samples, and they were placed in a boiling water bath for 60 min