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Deoxycholic acid, for example, signals through TGR5, leading to PKA inhibition and has been linked to EGFR activation, ERK1/2 signaling, and occludin redistribution in Caco-2 cells (57, 58)

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This dual-mechanism strategy enables researchers to explore the synergistic amplification of GH secretion, providing a powerful model for examining neuroendocrine regulation, metabolic processes, and tissue adaptation

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