Cytokine levels were measured using enzyme-linked immunosorbent (ELISA) assay kits: Human 1L-10 ELISA Ready-SET-Go from Affymetrix (Cat# 88-7106) and Human TNF- Uncoated ELISA (Cat # 88-7346), Human IFN- Uncoated ELISA (Cat # 88-7316), and Human IL-6 Uncoated ELISA (Cat # 88-7066) from Invitrogen to quantity detection of cytokines
The impact of AFB1 on the gut microbiota was evaluated by extracting the contents of the broiler chickens duodenum, following AFB1 exposure and using 16S rRNA sequencing to analyze the changes in abundance and diversity of the gut microbiota (Huang et al., 2023)
This leads scientists to hypothesize that increased food intake may be a factor in the observed weight gain among the Ipamorelin groups
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