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Immunoblot assays Cell lysates from HCC-1806, PC3 WT and PC3 GGT1 + cells were obtained by placing PBS-washed cell plates on ice and scraping with RIPA buffer (Thermo Scientific, 89900) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280)

Sacral nerve stimulation at subsensory threshold does not compromise treatment efficacy: results from a randomized, blinded crossover study

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