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doi: 10.1038/s41581-020-00367-2 20 MohiuddinMSHimenoTInoueRMiura-YuraEYamadaYNakai-ShimodaHet al

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Without enough glutathione, the liver can get "backed up" during this transition from Phase I to Phase II

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Readouts most often include glycerol release and free fatty acid quantification in the supernatant, alongside cellular ATP or viability measures to confirm that observed lipolysis reflects genuine metabolic signaling rather than cytotoxic membrane disruption