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The cells were cultured in Ultra-Low-Attachment plates (ULA) (Thermo Scientific) in PrimNeuS media ( 4.1.3 Mice and in vivo experiments All animal experiments were approved by The Institute of Cancer Research Animal Welfare and Ethical Review Body and performed in accordance with the UK Home Office Animals (Scientific Procedures) Act 1986, the UK National Cancer Research Institute guidelines for the welfare of animals in cancer research and the ARRIVE (animal research: reporting in-vivo experiments) guidelines
Also persistent diarrhoea sufferers
It does not affect insulin or IGF-1 levels and is well tolerated by most patients
It is used widely in pharma industry to ensure safety of the final product
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