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Antibodies used were as follows: FoxO3a (Cell Signaling Technology, #12829), phospho-FoxO3a (Ser253) (HUABIO, ST49-01), Atrogin-1 (Proteintech, 67172-1-Ig, RRID: AB_2882468), MuRF-1 (Proteintech, 55456-1-AP, RRID: AB_11182630), GAPDH (Proteintech, 60004-1-Ig, RRID: AB_2107436), and HRP-conjugated secondary antibodies (Proteintech, SA00001-1/SA00001-2)

10.1038/npp.2009.65 253 SesackS

Their physiology is different, their biology is different, and what is important from an outcome standpoint is different, as well, making it important not to try to extrapolate data from younger to older adults, he added

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