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Reconstitute AHK-Cu with SWFI or BAC water, then apply per protocol to cell cultures or tissue explants at concentrations typically ranging from 0.1 to 10 M

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2 g per well of cell membrane was incubated with furimazine (1:1,000 dilution from stock) in assay buffer (1 HBSS, 10 mM HEPES, 0.1% (w/v) BSA, 1 P8340 protease inhibitor cocktail, 1 mM DTT and 0.1 mM PMSF, pH 7.4)

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Then came the next wave: dual agonists