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Compared to normal joints, the extracellular matrix of RAM of RA boasts a higher-content of a series of inflammatory factors, matrix metalloproteinases (MMP), many kinds of RONS, H + , while simultaneously exhibiting a lower concentration of oxygen [7]

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Regarding static scaffold-free 3D cultures, the most common techniques employed are the forced floating method, the hanging drop method, and the magnetic levitation method while for dynamic systems, agitation-based techniques are applied using spinner flask bioreactors and rotational flask bioreactors [247]