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On day 21, tumor progression was evaluated using bioluminescence imaging with the IVIS system (Caliper Life Sciences) following intraperitoneal injection of D-Luciferin potassium salt (3 mg/kg per mouse, administered 5 minutes prior to imaging, Beyotime, ST198) and anesthesia with 1% pentobarbital sodium (80 mg/kg per mouse)

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10.1021/bi1004987

ANNs consist of interconnected nodes (neurons) organized in layers: input, hidden, and output layers, capable of capturing complex patterns in data

a regular brown network pattern with a homogenous blue area[61]