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Knisely AS, Strautnieks SS, Meier Y, Stieger B, Byrne JA et al

ORCID: Affiliation: 8 Neuroscience Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney NSW 2006, Australia 9 Laboratory of Neurobiology and Pathological Physiology, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 602 00 Brno, Czech Republic These authors contributed equally to this work

While ice numbs the skin temporarily, it also constricts blood vessels and increases tissue density, potentially making the injection more uncomfortable and slowing medication absorption

Statistical Analysis The data were analyzed using GraphPad Prism V7.0 (GraphPad 264 Software, Inc., San Diego, CA, United States)