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Blood samples were collected into anticoagulated tubes, centrifuged at 1000 rpm for 10 min at 4C, and the plasma samples were separated and stored in a 80C refrigerator

In brief, human umbilical vein endothelial cells (HUVECs) were seeded into 96-well culture plates (1 10 4 HUVECs/well) for 12 h, then the cells were changed to fresh medium containing drugs with different concentrations (6.25, 12.5, 25, 50 M)

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In like fashion, a case report suggested that FMT treatment of a patient with secondary progressive MS for Clostridium difficile enterocolitis correlated with disease stabilization (Makkawi et al., 2018)

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