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These results demonstrated the additive benefits of targeting both amylin and GLP-1 pathways for glycemic control and weight management in type 2 diabetes and supported advancement to Phase 3 development
Measurement of Blood Glucose Level Plasma glucose levels of animals in Experiment 2 were measured before SAH, at 0 (immediately), 30, 60, 90, 120, 180 min, and 24 h after delivering the vehicle or drug ( Subarachnoid Hemorrhage Grade Photographs of the basal surface of the brain sample were captured immediately after euthanasia and evaluated blindly, following a previously described procedure ( Neurological Evaluation Modified Garcia and the beam balance tests were performed to assess neurological function 24 h after SAH, as previously described ( Intracerebroventricular Administration Intracerebroventricular drug administration was performed as previously described ( (a) GLP-1R siRNA-1 Sense: GGCAUUGUCAAGUAUCUC UACGAGG Antisense: CCUCGUAGAGAUACUUGACAAUGCC (b) GLP-1R siRNA-2 Sense: GUUUCUGCCCUGCAUGG GUACUACT Antisense: AGUAGUACCCAUGCAGGGCAGAAAC (c) GLP-1R siRNA-3 Sense: AUAUCUGUGGAUGGUGUU GAAGATA Antisense: UAUCUUCAACACCAUCCCAGAUAT (d) Scrambled siRNA Sense: CGUUAAUCGCGUAUAAUA CGCGUAT Antisense: AUACGCGUAUUAUACGCGAU UAACGAC Dorsomorphin dihydrochloride (Santa Cruz Biotechnology Inc., TX, United States), a selective AMPK inhibitor, also known as compound C, was prepared at 10 mmol/L in 20% DMSO of PBS and injected (10 l) 30 min before SAH induction in SAH + Ex-4 + Dorsomorphin groups
doi: 10.1111/tra.12613 67 LiBMingHQinSNiceECDongJDuZet al
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