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However, because IGF-1 signaling operates through cellular proliferation pathways also implicated in oncogenic processes, published protocols consistently list active or precancerous conditions as contraindications requiring exclusion from study populations.

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demonstrated ZnO nanoparticles-mediated acute toxicological effect and developmental abnormalities in zebra fish embryo [334] due to DNA damage and apoptosis induction by p53-mitochondria-caspase mediated pathway