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The vital dye 2-(4-(dimethylamino)styryl)- N -ethylpyridinium iodide (DASPEI) (Life Technologies, Grand Island, NY) is a marker of mitochondrial membrane potential and preferentially stains lateral line hair cells when added to the surrounding EM (Harris et al., Aminoglycoside uptake Gentamicin conjugated to the fluorophore Texas Red (GTTR) was used to determine if Dihexa affected aminoglycoside uptake by hair cells (Steyger et al., Pharmacological inhibitors Pharmacological inhibition of HGF/c-Met or its downstream targets was used to elucidate which factors are important for Dihexa-mediated otoprotection

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