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Description

Gene silencing using siRNA and Western blot protocols are described in the Supplemental Methods, as is the use of streptavidin affinity pull-down of biotinylated peptides and MS

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Efficacy of black cohosh-containing preparations on menopausal symptoms: A meta-analysis

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The effect of hydrogen peroxide-induced oxidative stress on leukocytes depends on age and physical training in healthy human subjects carrying the same genotypes of antioxidant enzymes gene polymorphisms

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All samples were serially diluted in assay buffer in duplicate and incubated with cells for 30 min at RT before detection with the cAMP dynamic 2 HTRF assay (Cisbio assays, Bedford, MA) as described previously 51

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