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To assess downstream flux of valine or KIV into the TCA cycle, we measured enrichment of labeled succinate, malate, citrate, and -ketoglutarate (-KG) in the healthy and MMA hepatocytes incubated with labeled valine or KIV as substrates, with and without a range of BCATi concentrations (Figure 4A)

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Together, they form a powerful tool for researchers studying GH signaling, metabolic function, tissue repair, and cellular regeneration models

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