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DTC Experiments Cell lines derived from p48-Cre/p53F/+KrasL/+ (Sirt6 WT and KO) metastatic lung tumors (see above) were stably transduced with pLKO.1 vectors (Control and sh Gstt1 ) or iPCW-FLAG- Gstt1 (treated with doxycycline 5 days prior to injection) were harvested by trypsinization, washed twice in PBS, resuspended at 1 x 10 4 in 100ul PBS and injected into the tail vein of NOD.CB17-Prkdcscid/J mice (Jackson Laboratory) to establish lung metastases

These cells die off and your body will naturally expel them, resulting in fat reduction of up to 2025% in the targeted area

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