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Jegelka, S

Indeed, conducting NTA titration in 5 mM HEPES, pH 7.4 we observed that absence of 50 mM NaCl caused fivefold increase of the K D for CuHSA, which is, however, apparent and should be corrected by using of K D value for Cu(II)NTA complex at low ionic strength

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The 2 class of adrenergic receptors are coupled to G i -type G-proteins that inhibit the activation of adenylate cyclase and therefore, receptor activation results in reduced levels of cAMP and consequently reduced levels of active PKA