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For BSO group, a week later, mice received intraperitoneal administration of 750 mg/kg of body weight BSO (B113387, Aladdin) in phosphate-buffered saline (pH 7.4) once a day for two weeks 25

Red-flag symptoms gastrointestinal bleeding, unintentional weight loss, or a high fecal calprotectin always warrant conventional GI workup rather than a gut-repair protocol

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Covalent/irreversible inhibitors target the redox/catalytic apparatus of TGR with almost no recognition elements that give affinity and selectivity for TGR over off-targets and the cellular redox apparatus resulting in undesirable cross-reactions and low specificity in vivo 25

1 Averill-Bates DA