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To validate insertion at the molecular level, we extracted RNA from GFP-signal-positive tissue and performed reverse-transcription polymerase chain reaction (RT-PCR) using specific primers flanking the target site

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The annual Behavioral Risk Factors Survey conducted in house at DHS showed that adult smoking prevalence had increased from 16.7 percent to 18.6 percent between 1995 and 1996,61 reversing a downward trend that had existed for nearly a decade.24 62 The increase in smoking rates received wide media coverage.62-64 Public health groups blamed the increase on the fact that the administration had not fully funded Proposition 99's anti-tobacco education programs and on its reluctance to attack the tobacco industry

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In addition, Nepal has also seen a rampant growth of illegal production of SLT within the country in recent times 27