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This will allow for the pipe to properly soak rather than being faced with a barrier thats thicker than necessary

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After the transfer, membranes were blocked for 1 h in TBST and 5% non-fat milk and then incubated overnight with the primary antibody at 4C

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Chemical separation and identification of flavoring components via comprehensive HPLC and LCMS/GCMS analysis would be necessary to begin to determine any potential mechanisms of growth inhibition