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Many adult smokers use e-cigarettes as a harm-reduction tool to help transition away from combustible cigarettes, particularly when choosing regulated products like SMOKO

To make this possible, we need to modify the matrix transformation currently implemented in mmgsem package to enable the inclusion of observed items (Wall and Amemiya, 2000)

After four days of incubation at 37 C, 5% CO 2 , the plates were then centrifuged at 300 g in a Beckman TJ-6 centrifuge for 5 min and the supernatants collected for IFN analysis

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