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Genomic DNA was isolated from 1.8 2.0 ml EDTA whole blood samples using the Qiagen Blood Mini kit (Qiagen, Hilden, Germany) and 1.2 g were sent to Life & Brain GmbH (Bonn, Germany) for bisulfite conversion and subsequent analysis using the Infinium MethylationEPIC BeadChip (Illumina, San Diego, CA, USA)

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Instead of allowing the products to remain on the shelves during its pre-market review process, the FDA should order removal of all unapproved nicotine products, she said