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Cells were cultured at 37C in 5% CO 2 for another 18 h for reverse transcription-quantitative (RT-q) PCR and 48 h for western blot analysis

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This study also found a negative correlation between sperm mRNA levels of H2BFWT and non-progression/total motility while the transcript was positively correlate with non-motile sperm [97]

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