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Membranes were treated with 5% BSA in PBS-T (PBS with 0.1% Tween 20) blocking buffer for at least 1 h at room temperature, then incubated with primary antibody diluted in blocking buffer overnight at 4C

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Nicotine can have antihistamine-like or mast-cell-modulating effects in certain contexts, which is why some people with MCAS or histamine intolerance are curious about it

What you are actually smelling are the mycotoxins produced by mold