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The additive effects of nicotine on the severity of HFD-induced hepatic steatosis was associated with significantly greater oxidative stress, increased hepatic TG levels, higher incidence of hepatocellular apoptosis, inactivation (dephosphorylation) of AMPK, and activation of its downstream target ACC ( Figure 1 Indeed, these above studies, using various experimental models, demonstrated that nicotine further worsens HFD-induced hepatic steatosis

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